![]() ![]() ![]() This global phenomenon has been mainly associated with the dissemination of high-risk clones. Approximately 700,000 people die annually from antimicrobial-resistant infections, and this number is projected to surpass 10 million per year by 2050. The global spread of carbapenemase-producing (CP) Klebsiella pneumoniae (KPC) has become a major threat to human health. Keywords: Klebsiella pneumoniae, KPC-Kp ST307, bla KPC gene, horizontal transfer, Tn4401, glycogen synthesis This study provides a better understanding of the genetic characteristics that can contribute to the growth and spread of KPC-Kp ST307, and their association with antimicrobial resistance genes. Plasmids containing the bla KPC-2 gene could not be artificially removed thus, the three strains could not be cured.Ĭonclusions: The ease of horizontal transfer of KPC-Kp ST307 carbapenem resistance has serious public health and epidemiological implications. The bla KPC-2 gene was located on a truncated Tn4401 transposon. The plasmids could be transferred through conjugation, because all three strains of bacteria contained the type IV secretion system, pilus genes, and tra genes for conjugal transfer. pneumoniae ST307 was horizontally transmitted between these strains. pneumoniae (CPKp1825), Klebsiella aerogenes (CPEa1826), and Escherichia coli (CPEc1827). Three Enterobacteriaceae strains were identified in one patient: K. Results: Sequence analysis of KPC-Kp strain ST307 revealed novel plasmid-located virulence factors, including a gene cluster for glycogen synthesis. Methods: We performed antimicrobial susceptibility testing, PCR analysis, multilocus sequence typing, curing tests, and whole-genome sequencing to characterize plasmid-derived KPC-2-producing Enterobacteriaceae clinical isolates. pneumoniae (KPC-Kp) ST307, investigated the antimicrobial resistance conferred by this strain, and confirmed the horizontal interspecies transmission of KPC- carbapenemase-producing Enterobacteriaceae (CPE) characteristics among Enterobacteriaceae. We analyzed the complete sequences of plasmids from KPC-2-producing K. Background: A lineage of Klebsiella pneumoniae that produces carbapenemase-2 (KPC-2), sequence type (ST) 307, emerged in 2017. ![]()
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